This SOP follows the CCAC guidelines for acceptable injection volumes in rodents. This SOP follows the CCAC guidelines for the injection into the tail vein in laboratory rodents. During administration mice should be protected from pain, suffering, distress or lasting harm or at least pain and distress shall be kept to a minimum (ETS 123, 1986). CCAC guidelines on antibody production (2002) and Leenaars et al. Canadian Council on Animal Care (CCAC) guidelines and policies, and McGill University policies, procedures and guidelines. Two high-resolution (1920 × 1080) digital video cameras (High-Definition ccac rat guidelines 2020 (Apr 12, 2021) This SOP follows the CCAC guidelines for acceptable injection volumes For the Survival Bleeding of Mice and rats SOP animal care, Canada Manual Restraint and Common Compound Administration Routes in Housing and caretaking of the mice at the K.U.Leuven partner is in accordance with the Belgian and European laws, guidelines and policies for animal experimentation, housing and care (as documented in the most recent legislation, i.e. Responsibility In: ... An evaluation of distress following intraperitoneal immunization with Freund's adjuvant in mice. CCAC Guidelines on Antibody Production, Canadian Council on Animal Care (2002) Refinement and reduction in production of genetically modified mice (2003) Laboratory birds: refinements in husbandry and procedures (2001) Refining procedures for the administration of substances Principles of administration All animal experiments were performed according to protocol number A11-290, approved by the University of British Columbia's Animal Care Committee and in direct accordance with the Canadian Council of Animal Care (CCAC) guidelines. Responsibility Those trained persons listed on an approved Animal Care Committee protocol performing the procedure Subcutaneous (SC or SQ) Injection in Rats and Mice SOP Purpose This Standard Operating Procedure (SOP) describes the procedure of subcutaneous injections into rats and mice. Use committee and were consistent with Canadian Council on Animal Care guidelines.11 Digital video. the Belgian Royal Decree of 6 April 2010 and European Directive 2010/63/EU on the protection of animals used for scientific purposes of 20 October 2010). UBC Animal Care Committee; Policy on Acceptable Methods of Rodent Blood Withdrawal: ANIMAL CARE POLICIES, SOPs & GUIDELINES | Office of Research Services 3. handling and collection of blood samples from mice References: 1. in rats and mice using the lateral tail veins. Some injections (such as footpad injec-tion) are strongly discouraged and if required must be justified on a case by case basis (CCAC, 2002). Canadian Council on Animal Care (CCAC) guidelines (www.ccac.ca) 2. The CCAC is the national peer-review organization responsible for setting and maintaining standards for the ethical use and care of animals used in science throughout Canada. Canadian Council on Animal Care (1993). Recommendations ... still involves mice in the initial immunization stage, but in vitro techniques can be used for the propagation and harvesting of Mabs from the hybridoma—therefore causing significantly Along with frequently asked questions it provides a framework for current best practices with the aim of improving mouse welfare. CCAC guidelines on acceptable immunological procedures. Canadian Council on Animal Care (CCAC) guidelines (www.ccac.ca) Recommendations Use the manufacturer’s recommended route of injection since some drugs may have adverse side effects or cause discomfort if injected via a non‐recommended route. Mice were placed individually on a tabletop in cubicles (9 × 5 5 cm high) with 2 walls of transparent acrylic glass and 2 side walls of removable stainless steel. Laboratory Animal Science 39(2):122-126, ISSN:0023-6764. (1999). The Canadian Council on Animal Care (CCAC) document CCAC guidelines: Mice is now available.
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