For example, embryonic and adult heart rates are similar to those of humans, and action potential morphology and electrocardiogram (ECG) morphology also parallels those of humans. In particular, zebrafish display a robust T-type Ca2+ current in both atrial and ventricular cardiomyocytes. 8600 Rockville Pike Epub 2019 Jan 1. Animal heart models of human heart diseases are cornerstones of drug discovery, both to identify new drug targets and to screen new drugs for cardiovascular toxicities. Zebrafish as a model to study cardiac development and human cardiac disease. A heart attack is often a cause of heart muscle death, which is in turn a large contributor to disability. Heart Resection or Injury. An international research group now shows that certain heart muscle cells play a central role in this process. 11,12 Thus, zebrafish have become a popular model for the study of cardiac tissue regeneration. Interestingly, in most mammals T-type Ca2+ channels are only expressed in the developing heart or under pathophysiological conditions, indicating that adult zebrafish cardiomyocytes display a more immature phenotype. The zebrafish heart is similar to the human heart in many respects. Cardiovasc Res. Though distantly related, human and zebrafish hearts have much in common. Copyright © 2018 Elsevier Ltd. All rights reserved. Eighteen fish were divided into two arms: 6 sham operation and 12 apical ventricular resection. It turns out that zebrafish’s heart resembles that of the human heart so closely –70% of human genes are also found in the fish – it has made it a relevant and compelling model for research into how the heart develops and breaks. In this review, we will discuss the importance of in vitro model systems for cardiac research and summarise results of in vitro 3D heart-like cell aggregates, consisting of myocardial tissue formed spontaneously from enzymatically digested whole embryonic ZF larvae (Zebrafish Heart Aggregate - ZFHA). Gene mutations associated with cardiomyopathies can lead to various pathologies of heart failure. Despite the anatomical differences between fish and human hearts, similarities in cellular structure and conservation of genes as well as pathways across vertebrates have led to an … J Exp Biol. March 24, 2010 Zebrafish study with human heart implications: Cellular grown-ups outperform stem cells in cardiac repair. Whereas, humans cannot efficiently regenerate their heart after injury, the teleost zebrafish have the capability to repair a “broken” heart. ZF research benefits from an entirely sequenced genome, which allows us to establish and study cardiovascular mutants to better understand cardiovascular diseases. Lien CL, Harrison MR, Tuan TL, Starnes VA. Currently, approaches for discovering monogenetic factors involved in human congenital heart defects (CHDs) include pedigree studies and candidate screens. Prevention and treatment information (HHS). A multi-endpoint in vivo larval zebrafish (Danio rerio) model for the assessment of integrated cardiovascular function. In both cases, the heart is made up of chambers with valves that ensure blood flows in the correct direction. An electrophysiological study. 2014 Jan-Feb;69(1):30-8. doi: 10.1016/j.vascn.2013.10.002. Epub 2013 Oct 16. Generating an in vitro 3D cell culture model from zebrafish larvae for heart research. ZFHA have potential for the use of high-throughput-screenings of different factors like small molecules, nucleic acids, proteins and lipids which is difficult to achieve in the zebrafish in vivo screening models with lethal mutations as well as to explore ion channel disorders and to find appropriate drugs for safety screening. In a zebrafish, when that cell is damaged, it will activate and then regenerate. Privacy, Help We will give an overview of the similarities and differences of ZF versus human hearts and highlight why ZF complement established mammalian models (i.e. Twelve zebrafish underwent apical ventricular resection according to the previously described method. A whale of a heart. Adult zebrafish heart as a model for human heart? Spontaneously contracting cell aggregates derived from grass carp heart as a promising tool in in vitro heart research. Scientists use a variety of laboratory techniques to investigate the genetic cause of human diseases. 2019 Feb;71(1):261-266. doi: 10.1007/s10616-018-0281-x. 2012 Sep-Oct;20(5):638-46. doi: 10.1111/j.1524-475X.2012.00814.x. Despite the anatomical differences between fish and human hearts, similarities in cellular structure and conservation of genes as well as pathways across vertebrates have led to an increase in the popularity of ZF as a model for human cardiac research. Thus, zebrafish have been proposed as a cost-effective model for genetic and pharmacological screens of factors affecting heart function and repair. Keywords: 2011 Jul 15;91(2):279-88. doi: 10.1093/cvr/cvr098. Larval zebrafish have recently shown many advantages for human disease studies and drug discovery. 7 As in all vertebrates, the heart is the first organ to function in zebrafish and it develops rapidly … The zebrafish (Danio rerio) has become a popular model for human cardiac diseases and pharmacology including cardiac arrhythmias and its electrophysiological basis. Both are muscles designed to pump oxygen-carrying blood through the body. Salgado-Almario J, Vicente M, Vincent P, Domingo B, Llopis J. Int J Mol Sci. Wang decided to work with zebrafish as a postdoc at Duke with Kenneth Poss, who had shown in 2002 that zebrafish can recover after having substantial chunks of their heart muscles cut out. 2016). We show that zebrafish, like most mammals, display functional acetylcholine-activated K+ channels in the atrium, but not in the ventricle. Investigating aortic arch development. The physiology of the zebrafish heart has been suggested to resemble that of the human heart in many aspects, whereas, in contrast to mammals, the zebrafish has a remarkable ability to regenerate after heart injury. Parker T, Libourel PA, Hetheridge MJ, Cumming RI, Sutcliffe TP, Goonesinghe AC, Ball JS, Owen SF, Chomis Y, Winter MJ. However, despite these similarities between zebrafish and mammalian electrophysiology, we also identified important differences. Decellularised cardiac ECM from zebrafish has been shown to have a significant proliferative effect on human cardiac precursor cells and enables endogenous regeneration of mouse heart tissue post-MI (Chen et al. murine and large animal models) for cardiac research. Because of the embryo's small size, it does not need a working heart. Heart repair and regeneration: recent insights from zebrafish studies. Copyright © 2009 Elsevier Inc. All rights reserved. The embryonic zebrafish heart exhibits the same pattern of electrical excitation as the human heart, with pulses generated in the sino-atrial node, propagated through the … Unable to load your collection due to an error, Unable to load your delegates due to an error. The research shows, however, that gata5 can reprogramotherwise predestined embryonic cell types to become beating heart cells. It is already known that zebrafish can flexibly regenerate their hearts after injury. Here, we characterize action potentials (APs) from intact adult atria and ventricles and find that the overall shape of zebrafish APs is similar to that of humans. Heart defects are the most common congenital defect, yet scientists still struggle to understand why they occur. 3D culture; Cardiomyocyte; Heart electrophysiology; Safety pharmacology; Zebrafish mutants. By continuing you agree to the use of cookies. Grunow B, Wenzel J, Terlau H, Langner S, Gebert M, Kruse C. Cell Physiol Biochem. Improving drugs for irregular heart rhythms. 2020 Sep 10;21(18):6610. doi: 10.3390/ijms21186610. Heart failure results in the heart pumping blood incorrectly and in insufficient amounts throughout the body. LA JOLLA, CA—Bony fish like the tiny zebrafish have a remarkable ability that mammals can only dream of: if you lop off a chunk of their heart they swim sluggishly for a few days but within a month appear perfectly normal. 2019 Feb 28;7(1):15. doi: 10.3390/biomedicines7010015. And zebrafish hearts are simpler than mammals’: theirs have just two chambers, while ours have four. Zebrafish can heal their damaged hearts. Zebrafish embryos are particularly well-suited to investigating the mysteries of heart disorders, because even embryos that develop severely malformed, nonbeating hearts can survive and continue to grow for several days. Notably, the … If zebrafish gata5, or its human equivalent, could prompt a particular type of embryonic cell, known as a human embryonic stem cell, to become a beating heart cell, researchers could theoretically use this technique to cultivate and harvest such genetically modified cells in the petri dish and then transplant them into people with failing hearts. This site needs JavaScript to work properly. Zebrafish to model human congenital and acquired cardiac diseases 3.1 Congenital heart defects. Careers. The physiology of the zebrafish heart has been suggested to resemble that of the human heart in many aspects, whereas, in contrast to mammals, the zebrafish has a remarkable ability to regenerate after heart injury. However, the left half of the heart differs considerably from the right half. Epub 2011 May 19. Even though Zebrafish heart is different from human heart with only systemic circulation, heart structure and physiology are similar between the two species. Clipboard, Search History, and several other advanced features are temporarily unavailable. A new study from the Max Delbruck Center for Molecular Medicine investigates a signaling pathway in the developing heart that works similarly in both zebrafish … Although the zebrafish heart is two-chambered, its fundamental electrical properties are remarkably similar to those of human. It is still largely unclear how the two circulatory systems develop. The zebrafish - which can mend its own heart muscle - is providing clues to heart failure, which affects 750,000 people in the UK. The physiology of the zebrafish heart has been suggested to resemble that of the human heart in many aspects, whereas, in contrast to mammals, the zebrafish has a … However, realizing the full potential of the zebrafish heart as a model will require a better understanding of the electrophysiology of the adult zebrafish myocardium. The zebrafish is probably one of the most important models for developmental and regenerative biology of the heart. Two months later, the zebrafish has completely regenerated its heart. At this stage, the ZFHA model system is being refined into a high-throughput (more ZFHA generated than larvae prepared) and stable in vitro test system to accomplish the same longevity of previously successful salmonid models. National Library of Medicine Epub 2011 Feb 11. Humans hearts retain scars of earlier injuries. Journal of Molecular and Cellular Cardiology, https://doi.org/10.1016/j.yjmcc.2009.08.034. J Pharmacol Toxicol Methods. Would you like email updates of new search results? Thanks to an almost entirely sequenced genome and high conservation of gene function compared with humans, the zebrafish has proved highly informative to express and study human disease-related gene variants, providing novel insights into human cardiovascular disease mechanisms, and highlighting the suitability of the zebrafish as an excellent model to study human cardiovascular diseases. "It is the size of a small car and has been weighed at about 950 pounds [430 kg]," … 6 Zebrafish heart is highly comparable with human heart in structures, functions, signal pathways, and ion channels, and both are muscles designed to pump oxygen-carrying blood through the body. On Zebrafish Disease Models and Matters of the Heart. Zebrafish: A model for heart development. While the zebrafish heart, which is comprised of a single ventricle and atria, is a simpler version of the human heart, the mechanisms regulating its development share much in common. The zebrafish (ZF) has become an essential model for biomedical, pharmacological and eco-toxicological heart research. In contrast, adult zebrafish (Danio rerio) efficiently and completely regenerate working heart tissue via hyperplasia—proliferation of existing cardiac cells. Rastgar S, Alijani Ardeshir R, Movahedinia A, Zabihi E, Salati AP, Salamat N. Cytotechnology. The zebrafish (ZF) has become an essential model for biomedical, pharmacological and eco-toxicological heart research. How is your heart like that of a zebrafish? The largest animal heart is the blue whale’s, which has been weighed at about 400 pounds (and it is not the size of a small car, contrary to popular belief). Epub 2015 Feb 24. The zebrafish has recently emerged as an excellent model for studies of heart development and regeneration. The human heart is about the size of a fist — and a cow’s heart is the size of a human head. 2015 Apr 15;218(Pt 8):1116-21. doi: 10.1242/jeb.118224. When a heart beats irregularly, it can increase the risk … Biomedicines. 2011;27(1):1-12. doi: 10.1159/000325212. Wound Repair Regen. Epub 2012 Jul 20. Furthermore, the zebrafish AP upstroke is dominated by Na+ channels, L-type Ca2+ channels contribute to the plateau phase and IKr channels are involved in repolarization. 24 Zebrafish were sedated in 5% Tricaine methanesulfonate (Tricaine). March 24, 2010. Through their studies of zebrafish, scientists at the Max Planck Institute for Heart and Lung Research have found that the mammalian heart is an evolutionary development of the fish heart. Zebrafish hearts are surprisingly similar to those of humans, making them useful in cardiac research. FOIA "So, the fish will go from blind to about 2 1/2 weeks later, total regain of eyesight," Patton said. Embryonic Zebrafish has emerged more recently for cardiovascular research to be used in high‐throughput studies. In vitro developed spontaneously contracting cardiomyocytes from rainbow trout as a model system for human heart research. 3. Copyright © 2021 Elsevier B.V. or its licensors or contributors. Research often utilizes patients’ cells or tissue samples, but to determine if a mutation in a specific gene can cause a patient’s symptoms, we often need experimental animal models. Please enable it to take advantage of the complete set of features! Bethesda, MD 20894, Copyright Accessibility In less than four decades since Streisinger et al. If only we could do that. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. Heart failure is a complex pathophysiological syndrome of pumping functional failure that results from injury, infection or toxin-induced damage on the myocardium, as well as genetic influence. We use cookies to help provide and enhance our service and tailor content and ads. Mapping Calcium Dynamics in the Heart of Zebrafish Embryos with Ratiometric Genetically Encoded Calcium Indicators.
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